Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-contain ing wastewaters using Liquid Chromatography- Mass Spectrometry/Mass Spectrometry (LC- MS/MS)

摘要

Biologicaltreatmentsto degradecyanidearea powerfultechnologyforcyanideremovalfromindustrialwastewaters.It hasbeenpreviouslydemonstratedthatthealkaliphilicbacteriumPseudomonaspseudoalcaligenesCECT5344is ableto usefreecyanideandseveralmetal−cyanidecomplexesasthesolenitrogensource.In thiswork,thestrainCECT5344hasbeenusedfordetoxificationof thedifferentchemicalformsof cyanidethatarepresentin alkalinewastewatersfromthejewelryindustry.Thisliquidresiduealsocontainslargeconcentrationsof metalslikeiron,copperandzinc,makingthiswastewaterevenmoretoxic.Toelucidatethemolecularmechanismsinvolvedin thebioremediationprocess,a quantitativeproteomicanal-ysisbyLC-MS/MShasbeencarriedoutinP.pseudoalcaligenesCECT5344cellsgrownwiththejewelryresidueassolenitrogensource.Differentproteinsrelatedto cyanideandcyanateassimilation,aswellasotherproteinsinvolvedin transportandresistanceto metalswereinducedbythecyanide-containingjewelryresidue.GntR-likeregulatoryproteinswerealsoinducedbythisindustrialresidueandmutationalanalysisrevealedthatGntR-likeregulatoryproteinsmayplaya rolein theregulationof cyanideassimilationinP.pseudoalcaligenesCECT5344.ThestrainCECT5344hasbeenusedin a batchreactorto removeat pH9 thedif-ferentformsof cyanidepresentin industrialwastewatersfromthejewelryindustry(0.3g/L,ca.12mMtotalcyanide,includingbothfreecyanideandmetal−cyanidecomplexes).Thisisthefirstreportdescribingthebiologicalremovalat alkalinepHof suchaselevatedconcentra-tionof cyanidepresentin a heterogeneous mixturefromanindustrialsource.